Effects of natamycin and Lactobacillus buchneri on the fermentative process and aerobic stability of maize silage

The present study was aimed to evaluate the reduction in fermentative losses and the improvement of aerobic stability of maize silage treated with Lactobacillus buchneri bacteria, antifungal natamycin and a combination of L. buchneri and natamycin. The study was completely randomized using four treatments with four replicates (silo) each. The treatments were as follows: C - control (forage without additives), NA - forage with low dose of natamycin (8 g/t) addition, LB - forage inoculated with low dose of L. buchneri (5 × 104 cfu/g) and NLB - forage treated with both natamycin (8 g/t) and L. buchneri (5 × 104 cfu/g). The losses of dry matter (DM) and gas, effluent production, chemical composition, yeast count and aerobic stability were calculated for all treatments. During fermentation, NLB produced more propionic and lactic acids and caused less DM and gas losses than other treatments (P < 0.01). The positive effect of NLB on yeast inhibition improved the aerobic stability of maize silage (P < 0.05). Thus, the combination of low doses of natamycin and heterolactic bacteria L. buchneri can reduce fermentative losses and improve the aerobic stability of maize silage after exposure to air. © 2020 Polish Academy of Science. All rights reserved

A rapid staining technique for the detection of the initiation of germination of bacterial spores

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/75645/1/j.1472-765x.2002.01047.x.pd

How Has COVID-19 Affected Our Orthopedic Implant Industry Partners? Implications for the Surgeon-Industry Relationship in 2020 and Beyond

This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.Background The COVID-19 pandemic has had far-reaching societal and financial consequences. The purpose of this study was to evaluate how COVID-19 has affected AAHKS industry partners and the surgeon-industry relationship, emphasizing education, resource allocation, and strategic direction for the 2nd half of 2020. Methods AAHKS industry partners were contacted to participate in a blinded survey and optional interview with the AAHKS Industry Relations Committee. Based on the results, a group of AAHKS member surgeons with disparate practice types were asked to postulate on how the COVID-19 pandemic has and will affect their practice and relationship with Industry. Results AAHKS industry partner responses indicated decreased resource allocation for regional, “other national,” and AAHKS annual meetings (67%, 55%, and 30%, respectively). Web-based educational content was expected to increase in 2020 and will likely remain a point of emphasis in 2021 (100% and 70% of responders). For Q3/Q4 2020, a significant emphasis was placed on site of service/outpatient TJA and COVID-19-related safety measures (70% and 90% of responders), as well as increased availability of instrumentation and implants (40% and 60%, respectively). Conclusion The COVID-19 pandemic has altered the orthopedic landscape for the foreseeable future. Survey responses by AAHKS industry partners demonstrate a continued commitment to surgeon education with and increasing shift to a web-based platform. Increased resource allocation for outpatient TJA and COVID-19-related safety measures were significant. Articulating optimal mechanisms to aid industry in supporting surgeons with different practice models to meet demand during the second half of fiscal year 2020 will be critical

Experimental and modeling study of the autoignition of 1-hexene/iso-octane mixtures at low temperatures

Autoignition delay times have been measured in a rapid compression machine at Lille at temperatures after compression from 630 to 840 K, pressures from 8 to 14 bar, \Phi = 1 and for a iso octane/1 hexene mixture containing 82% iso-octane and 18% 1 hexene. Results have shown that this mixture is strongly more reactive than pure iso-octane, but less reactive than pure 1 hexene. It exhibits a classical low temperature behaviour, with the appearance of cool flame and a negative temperature coefficient region. The composition of the reactive mixture obtained after the cool flame has also been determined. A detailed kinetic model has been obtained by using the system EXGAS, developed in Nancy for the automatic generation of kinetic mechanisms, and an acceptable agreement with the experimental results has been obtained both for autoignition delay times and for the distribution of products. A flow rate analysis reveals that the crossed reactions between species coming from both reactants (like H-abstractions or combinations) are negligible in the main flow consumption of the studied hydrocarbons. The ways of formation of the main primary products observed and the most sensitive rate constants have been identified

Genetic Variation in KCNQ1 Associates With Fasting Glucose andβ-Cell Function: A Study of 3,734 Subjects Comprising Three Ethnicities Living in Singapore

10.2337/db08-1138Diabetes5861445-1449DIAE

CARACTERIZAÇÃO SOROLÓGICA DOS ANTÍGENOS DE SUPERFÍCIE EM CEPAS DE ESCHERICHIA COLI ISOLADAS DE SUÍNOS COM DIARRÉIA NO ESTADO DO PARANÁ

The aim of the present work wasthe serological characterization of 42 strains ofEscherichia coli isolated at the Center of VeterinaryDiagnostic Marcos Enrietti, in Curitiba, PR from outbreaks of swine diarrhea in the State of Paraná. Thesurface O-K and adherence antigens were identifiedat the Center of Veterinary Researches DesidérioFinamor (RS) and at UNICAMP (SP), respectively.The main serogroups involved were O138:K81 andO141:K85 which occurred in the same proportion in9 (21.4%) strains; 4 (9.5%) strains were O139:K82and 3 (7.1%) were O149:K91. The serogroupsO35:K”V79”, O108:K”V189”, O115:K”V165” andO119:K”V113” were found in only one sample each(2.4%) of the strains. In relation to fimbrial antigens,K88 was detected in 22 (52.4%) strains and theF165 in 5 (11.9%) strains. This is the first report ofthe occurrence of the fimbrial antigen named F165in Brazil. It was concluded that the inclusion ofthese more prevalent serogroups O-K as well as theclassical fimbriae and the F165 antigen could givemore protection against the colibacillosis of pigletsin Parana State.O presente trabalho teve como objetivoa caracterização sorológica de 42 cepas deEscherichia coli isoladas no Centro de DiagnósticoMarcos Enrietti em Curitiba, PR de surtos de diarréiasuína no Estado do Paraná. Os antígenos desuperfície O-K e de aderência foram identificadosno Centro de Pesquisas Veterinárias Desidério Finamor(RS), e na UNICAMP (SP), respectivamente.Os principais sorogrupos mais prevalentes foramO138:K81 e O141:K85, ocorrendo na mesmaproporção, em 9 (21,4%) cepas; O139:K82 em 4(9,5%) e O149:K91 em 3 (7,1%) totalizando 25(59,4%) cepas. Os sorogrupos menos prevalentestotalizaram 4 (9,5%) cepas e foram identificadoscomo O35:K”V79”, O108:K”V189”, O115:K”V165” eO119:K”V113”. Quanto aos antígenos de aderência,a fímbria K88 foi detectada em 22 (52,4%) cepas. Oantígeno F165 ocorreu em 5 (11,9%) cepas sendoeste o primeiro relato da ocorrência deste antígenoem cepas ETEC infectando suínos no Brasil. Baseadono levantamento sorológico concluiu-se quea inclusão nas vacinas dos sorogrupos O-K maisprevalentes e dos antígenos de aderência clássicosbem como do F165 poderá conferir proteção maisefetiva na prevenção da colibacilose dos leitões noEstado do Paraná

Coarsening Dynamics of Domains in Lipid Membranes

We investigate isothermal diffusion and growth of micron-scale liquid domains within membranes of free-floating giant unilamellar vesicles with diameters between 80 and 250 Am. Domains appear after a rapid temperature quench, when the membrane is cooled through a miscibility phase transition such that coexisting liquid phases form. In membranes quenched far from a miscibility critical point, circular domains nucleate and then progress within seconds to late stage coarsening in which domains grow via two mechanisms 1), collision and coalescence of liquid domains, and 2), Ostwald ripening. Both mechanisms are expected to yield the same growth exponent, alpha = 1/3, where domain radius grows as time(alpha). We measure alpha = 0.28 +/- 0.05, in excellent agreement. In membranes close to a miscibility critical point, the two liquid phases in the membrane are bicontinuous. A quench near the critical composition results in rapid changes in morphology of elongated domains. In this case, we measure alpha = 0.50 +/- 0.16, consistent with theory and simulation